rrid ab 628073 rabbit anti hb egf bioss bs 3576r  (Bioss)

Journal: Laryngoscope Investigative Otolaryngology

Article Title: Analysis of the epidermal growth factor receptor/phosphoinositide‐dependent protein kinase‐1 axis in tumor of the external auditory canal in response to epidermal growth factor stimulation

doi: 10.1002/lio2.785

Figure Lengend Snippet: Antibodies used for immunostaining

Article Snippet: EGF, 90‐140/208 , Bioss Antibodies, Woburn, MA, rabbit, bs‐3576R , 1:200 , Primary.

Techniques:

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: HB-EGF Plays a Pivotal Role in Mucosal Hyperplasia During Otitis Media Induced by a Viral Analog

doi: 10.3389/fcimb.2022.823714

Figure Lengend Snippet: Western blotting demonstrates HB-EGF processing during OM. (A) Representative blots for rat MEs are shown. The processed form, sHB-EGF, was distinguished from proHB-EGF by its lower molecular weight, (B) Relative band intensity quantification confirms that sHB-EGF increases to a maximum 2 days after administration of 500 μg of Poly(I:C), then progressively decreases. Saline control mucosa at 2 days shows a low sHB-EGF level. N refers to number of samples. *p < 0.05, **p < 0.01.

Article Snippet: After quenching endogenous peroxidases, sections were incubated with anti-HB-EGF antibody (rabbit polyclonal, 1:200, Bioss) that recognizes both forms of the factor for 24 hours.

Techniques: Western Blot, Molecular Weight

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: HB-EGF Plays a Pivotal Role in Mucosal Hyperplasia During Otitis Media Induced by a Viral Analog

doi: 10.3389/fcimb.2022.823714

Figure Lengend Snippet: An HB-EGF inhibitor decreases mucosal thickness during OM. Poly(I:C)-induced hyperplasia of rat ME mucosa was inhibited by co-administration of CRM197. (A) Representative microphotographs are shown on day 2. Scale bar = 100 μm. (B) Mean thickness of saline ME mucosa versus 500 ug of Poly(I:C), without or with various levels of CRM197 on day 2. N refers to number of MEs. *p < 0.05; **p < 0.01.

Article Snippet: After quenching endogenous peroxidases, sections were incubated with anti-HB-EGF antibody (rabbit polyclonal, 1:200, Bioss) that recognizes both forms of the factor for 24 hours.

Techniques:

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: HB-EGF Plays a Pivotal Role in Mucosal Hyperplasia During Otitis Media Induced by a Viral Analog

doi: 10.3389/fcimb.2022.823714

Figure Lengend Snippet: CRM197 does not inhibit HB-EGF expression or processing. (A) Representative microphotographs of immunostaining with a pan anti-HB-EGF antibody are shown. HB-EGF was expressed in rat ME mucosa in all 3 groups on day 2. (B) Representative Western blot sHB-EGF bands for rat ME, 2 days after saline injection or Poly(I:C) administration with or without CRM197. (C) Quantitative analysis showed that sHB-EGF was low in the saline samples but was increased by both Poly(I:C) and Poly(I:C) plus CRM197. N refers to number of samples. *p < 0.05.

Article Snippet: After quenching endogenous peroxidases, sections were incubated with anti-HB-EGF antibody (rabbit polyclonal, 1:200, Bioss) that recognizes both forms of the factor for 24 hours.

Techniques: Expressing, Immunostaining, Western Blot, Injection